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Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.


ABSTRACT: A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors.

SUBMITTER: Nozawa-Inoue M 

PROVIDER: S-EPMC2268300 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.

Nozawa-Inoue Mamie M   Jien Mercy M   Hamilton Nicholas S NS   Stewart Valley V   Scow Kate M KM   Hristova Krassimira R KR  

Applied and environmental microbiology 20080201 6


A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reducing bacterial communities following exposure to different electron donors. ...[more]

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