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Cost-effective production of a vaginal protein microbicide to prevent HIV transmission.


ABSTRACT: A series of small-molecule microbicides has been developed for vaginal delivery to prevent heterosexual HIV transmission, but results from human clinical trials have been disappointing. Protein-based microbicides, such as HIV-specific monoclonal antibodies, have been considered as an alternative approach. Despite their promising safety profile and efficacy, the major drawback of such molecules is the economy of large-scale production in mammalian cells, the current system of choice. Here, we show that an alternative biomanufacturing platform is now available for one of the most promising anti-HIV antibodies (2G12). Our data show that the HIV-neutralization capability of the antibody is equal to or superior to that of the same antibody produced in CHO cells. We conclude that this protein production system may provide a means to achieve microbicide ingredient manufacture at costs that would allow product introduction and manufacture in the developing world.

SUBMITTER: Ramessar K 

PROVIDER: S-EPMC2268773 | biostudies-literature | 2008 Mar

REPOSITORIES: biostudies-literature

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Cost-effective production of a vaginal protein microbicide to prevent HIV transmission.

Ramessar Koreen K   Rademacher Thomas T   Sack Markus M   Stadlmann Johannes J   Platis Dimitris D   Stiegler Gabriela G   Labrou Nikos N   Altmann Fritz F   Ma Julian J   Stöger Eva E   Capell Teresa T   Christou Paul P  

Proceedings of the National Academy of Sciences of the United States of America 20080303 10


A series of small-molecule microbicides has been developed for vaginal delivery to prevent heterosexual HIV transmission, but results from human clinical trials have been disappointing. Protein-based microbicides, such as HIV-specific monoclonal antibodies, have been considered as an alternative approach. Despite their promising safety profile and efficacy, the major drawback of such molecules is the economy of large-scale production in mammalian cells, the current system of choice. Here, we sho  ...[more]

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