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Substrate specificity of human kallikreins 1 and 6 determined by phage display.


ABSTRACT: The human tissue kallikrein (KLK) family contains 15 secreted serine proteases that are expressed in a wide range of tissues and have been implicated in different physiological functions and disease states. Of these, KLK1 has been shown to be involved in the regulation of multiple physiological processes such as blood pressure, smooth muscle contraction, and vascular cell growth. KLK6 is overexpressed in breast and ovarian cancer tissues and has been shown to cleave peptide derived from human myelin protein and Abeta amyloid peptide in vitro. Here we analyzed the substrate specificity of KLK1 and KLK6, by substrate phage display using a random octapeptide library. Consistent with earlier biochemical data, KLK1 was shown to exhibit both trypsin- and chymotrypsin-like selectivities with Tyr/Arg preferred at site P1, Ser/Arg strongly preferred at P1', and Phe/Leu at P2. KLK6 displayed trypsin-like activity, with the P1 position occupied only by Arg and a strong preference for Ser in P1'. Docking simulations of consensus peptide provide information on the identity of the enzyme residues that are responsible for substrate binding. Bioinformatic analysis suggested several putative KLK6 protein substrates, such as ionotropic glutamate receptor (GluR) and synphilin.

SUBMITTER: Li HX 

PROVIDER: S-EPMC2271166 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Substrate specificity of human kallikreins 1 and 6 determined by phage display.

Li Hai-Xin HX   Hwang Bum-Yeol BY   Laxmikanthan Gurunathan G   Blaber Sachiko I SI   Blaber Michael M   Golubkov Pavel A PA   Ren Pengyu P   Iverson Brent L BL   Georgiou George G  

Protein science : a publication of the Protein Society 20080401 4


The human tissue kallikrein (KLK) family contains 15 secreted serine proteases that are expressed in a wide range of tissues and have been implicated in different physiological functions and disease states. Of these, KLK1 has been shown to be involved in the regulation of multiple physiological processes such as blood pressure, smooth muscle contraction, and vascular cell growth. KLK6 is overexpressed in breast and ovarian cancer tissues and has been shown to cleave peptide derived from human my  ...[more]

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