Human UGT1A8 and UGT1A10 mRNA are expressed in primary human hepatocytes.
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ABSTRACT:
SUBMITTER: Li X
PROVIDER: S-EPMC2275121 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
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Human UGT1A8 and UGT1A10 mRNA are expressed in primary human hepatocytes.
Li Xin X Bratton Stacie S Radominska-Pandya Anna A
Drug metabolism and pharmacokinetics 20070601 3
It is widely believed that the UGT1A isoforms, UGT1A8 and -1A10, are expressed exclusively in extrahepatic tissues. In this work, human primary hepatocytes from six donors were analyzed for UGT1A8 and -1A10 mRNA expression by semi-quantitative RT-PCR. New primers to amplify UGT1A8 mRNA were designed and found to differ from those previously published. We demonstrated that UGT1A8 and -1A10 mRNA are expressed in hepatocytes. Although basal UGT mRNA levels were detected in untreated hepatocytes, si ...[more]
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