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Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors.

ABSTRACT: Intraflagellar transport (IFT) of a approximately 17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP tagged IFT proteins. Endogenous IFT proteins are most highly concentrated within the inner segment, around the basal body, and within the outer segment IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus.

SUBMITTER: Luby-Phelps K

PROVIDER: S-EPMC2276311 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature

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Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors.

Luby-Phelps Katherine K Fogerty Joseph J Baker Sheila A SA Pazour Gregory J GJ Besharse Joseph C JC

Vision research 20071010 3

Intraflagellar transport (IFT) of a approximately 17S particle containing at least 16 distinct polypeptides is required for the assembly and maintenance of cilia and flagella. Although both genetic and biochemical evidence suggest a role for IFT in vertebrate photoreceptors, the spatial distribution of IFT proteins within photoreceptors remains poorly defined. We have evaluated the distribution of 4 IFT proteins using a combination of immunocytochemistry and rod-specific overexpression of GFP ta ...[more]

PMID: 17931679

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