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Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia.

ABSTRACT: The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.

SUBMITTER: Hou XL

PROVIDER: S-EPMC2276671 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia.

Hou Xiao-li XL Jiang Han-liang HL Cao Qing-yi QY Zhao Li-ying LY Chang Barbara J BJ Chen Zhi Z

Journal of Zhejiang University. Science. B 20080401 4

The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging ...[more]

PMID: 18381803

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