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Structure-system correlation identifies a gene regulatory Mediator submodule.

ABSTRACT: A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/18/20 as a predominantly positive, gene-specific submodule required for low transcription levels of nonactivated genes, including conjugation genes. The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation.

SUBMITTER: Lariviere L

PROVIDER: S-EPMC2279198 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Structure-system correlation identifies a gene regulatory Mediator submodule.

Larivière Laurent L Seizl Martin M van Wageningen Sake S Röther Susanne S van de Pasch Loes L Feldmann Heidi H Strässer Katja K Hahn Steve S Holstege Frank C P FC Cramer Patrick P

Genes & development 20080401 7

A combination of crystallography, biochemistry, and gene expression analysis identifies the coactivator subcomplex Med8C/18/20 as a functionally distinct submodule of the Mediator head module. Med8C forms a conserved alpha-helix that tethers Med18/20 to the Mediator. Deletion of Med8C in vivo results in dissociation of Med18/20 from Mediator and in loss of transcription activity of extracts. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome, establishing Med8C/ ...[more]

PMID: 18381891

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