On the analysis of membrane protein circular dichroism spectra.

ABSTRACT: Analysis of circular dichroism spectra of proteins provides information about protein secondary structure. Analytical methods developed for such an analysis use structures and spectra of a set of reference proteins. The reference protein sets currently in use include soluble proteins with a wide range of secondary structures, and perform quite well in analyzing CD spectra of soluble proteins. The utility of soluble protein reference sets in analyzing membrane protein CD spectra, however, has been questioned in a recent study that found current reference protein sets to be inadequate for analyzing membrane proteins. We have examined the performance of reference protein sets available in the CDPro software package for analyzing CD spectra of 13 membrane proteins with available crystal struct

SUBMITTER: Sreerama N 

PROVIDER: S-EPMC2286510 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature