Ontology highlight
ABSTRACT:
SUBMITTER: Choi KH
PROVIDER: S-EPMC2286544 | biostudies-literature | 2004 Oct
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20041001 10
Bovine viral diarrhea virus (BVDV) nonstructural protein 5B is an RNA-dependent RNA polymerase, essential for viral replication. Initial attempts to crystallize a soluble form of the 695-residue BVDV polymerase did not produce any crystals. Limited proteolysis, homology modeling, and mutagenesis data were used to aid the design of polymerase constructs that might crystallize more readily. Limited proteolysis of the polymerase with trypsin identified a domain boundary within the protein. Homology ...[more]