The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila.
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ABSTRACT:
SUBMITTER: Makokha M
PROVIDER: S-EPMC2286742 | biostudies-literature | 2004 Mar
REPOSITORIES: biostudies-literature
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The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila.
Makokha Moses M Huang Yuanpeng Janet YJ Montelione Gaetano G Edison Arthur S AS Barbar Elisar E
Protein science : a publication of the Protein Society 20040206 3
The structure of Drosophila LC8 pH-induced monomer has been determined by NMR spectroscopy using the program AutoStructure. The structure at pH 3 and 30 degrees C is similar to the individual subunits of mammalian LC8 dimer with the exception that a beta strand, which crosses between monomers to form an intersubunit beta-sheet in the dimer, is a flexible loop with turnlike conformations in the monomer. Increased flexibility in the interface region relative to the rest of the protein is confirmed ...[more]
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