Ontology highlight
ABSTRACT:
SUBMITTER: Havens AM
PROVIDER: S-EPMC2288546 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Havens Aaron M AM Pedersen Elisabeth A EA Shiozawa Yusuke Y Ying Chi C Jung Younghun Y Sun Yanxi Y Neeley Chris C Wang Jincheng J Mehra Rohit R Keller Evan T ET McCauley Laurie K LK Loberg Robert D RD Pienta Kenneth J KJ Taichman Russell S RS
Neoplasia (New York, N.Y.) 20080401 4
We developed a sensitive real-time polymerase chain reaction (QPCR) assay that allows us to track early lodging/homing events in vivo. We used this technology to develop a metastasis assay of human prostate cancer (PCa) growth in severe combined immunodeficient mice. For this purpose, marked human PCa cell lines were implanted subcutaneously or in the prostate (orthotopically) of severe combined immunodeficient mice as models of primary tumors. Mice were then sacrificed at various time points, a ...[more]