Unknown

Dataset Information

0

Restricted expression of Epstein-Barr virus latent genes in murine B cells derived from embryonic stem cells.


ABSTRACT: Several human malignancies are associated with Epstein-Barr virus (EBV) and more than 95% of the adult human population carries this virus lifelong. EBV efficiently infects human B cells and persists in this cellular compartment latently. EBV-infected B cells become activated and growth transformed, express a characteristic set of viral latent genes, and acquire the status of proliferating lymphoblastoid cell lines in vitro. Because EBV infects only primate cells, it has not been possible to establish a model of infection in immunocompetent rodents. Such a model would be most desirable in order to study EBV's pathogenesis and latency in a suitable and amenable host.We stably introduced recombinant EBV genomes into mouse embryonic stem cells and induced their differentiation to B cells in vitro to develop the desired model. In vitro differentiated murine B cells maintained the EBV genomes but expression of viral genes was restricted to the latent membrane proteins (LMPs). In contrast to human B cells, EBV's nuclear antigens (EBNAs) were not expressed detectably and growth transformed murine B cells did not arise in vitro. Aberrant splicing and premature termination of EBNA mRNAs most likely prevented the expression of EBNA genes required for B-cell transformation.Our findings indicate that fundamental differences in gene regulation between mouse and man might block the route towards a tractable murine model for EBV.

SUBMITTER: Zychlinska M 

PROVIDER: S-EPMC2289878 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Restricted expression of Epstein-Barr virus latent genes in murine B cells derived from embryonic stem cells.

Zychlinska Magdalena M   Herrmann Heidrun H   Zimber-Strobl Ursula U   Hammerschmidt Wolfgang W  

PloS one 20080416 4


<h4>Background</h4>Several human malignancies are associated with Epstein-Barr virus (EBV) and more than 95% of the adult human population carries this virus lifelong. EBV efficiently infects human B cells and persists in this cellular compartment latently. EBV-infected B cells become activated and growth transformed, express a characteristic set of viral latent genes, and acquire the status of proliferating lymphoblastoid cell lines in vitro. Because EBV infects only primate cells, it has not b  ...[more]

Similar Datasets

| S-EPMC3541792 | biostudies-literature
| S-EPMC7673117 | biostudies-literature
| S-EPMC5762840 | biostudies-literature
| S-EPMC3993812 | biostudies-literature
| S-EPMC9927364 | biostudies-literature
| S-EPMC10376452 | biostudies-literature
| S-EPMC6435433 | biostudies-literature
| S-EPMC4273190 | biostudies-literature
| S-EPMC1544174 | biostudies-literature
| S-EPMC5309966 | biostudies-other