Identification and characterization of an archaeon-specific riboflavin kinase.
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ABSTRACT:
SUBMITTER: Mashhadi Z
PROVIDER: S-EPMC2293203 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Publications
Identification and characterization of an archaeon-specific riboflavin kinase.
Mashhadi Zahra Z Zhang Hong H Xu Huimin H White Robert H RH
Journal of bacteriology 20080201 7
The riboflavin kinase in Methanocaldococcus jannaschii has been identified as the product of the MJ0056 gene. Recombinant expression of the MJ0056 gene in Escherichia coli led to a large increase in the amount of flavin mononucleotide (FMN) in the E. coli cell extract. The unexpected features of the purified recombinant enzyme were its use of CTP as the phosphoryl donor and the absence of a requirement for added metal ion to catalyze the formation of FMN. Identification of this riboflavin kinase ...[more]
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