Ontology highlight
ABSTRACT:
SUBMITTER: Hvastkovs EG
PROVIDER: S-EPMC2323608 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
Analytical chemistry 20070130 5
Arrays suitable for genotoxicity screening are reported that generate metabolites from cytochrome P450 enzymes (CYPs) in thin-film spots. Array spots containing DNA, various human cyt P450s, and electrochemiluminescence (ECL) generating metallopolymer [Ru(bpy)2PVP10]2+ were exposed to H2O2 to activate the enzymes. ECL from all spots was visualized simultaneously using a CCD camera. Using benzo[a]pyrene as a test substrate, enzyme activity for producing DNA damage in the arrays was found in the o ...[more]