Ontology highlight
ABSTRACT:
SUBMITTER: Williamson MP
PROVIDER: S-EPMC2323994 | biostudies-literature | 2003 Sep
REPOSITORIES: biostudies-literature
Williamson Michael P MP Akasaka Kazuyuki K Refaee Mohamed M
Protein science : a publication of the Protein Society 20030901 9
The solution structure of bovine pancreatic trypsin inhibitor (BPTI) at a pressure of 2 kbar is presented. The structure was calculated as a change from an energy-minimized low-pressure structure, using (1)H chemical shifts as restraints. The structure has changed by 0.24 A RMS, and has almost unchanged volume. The largest changes as a result of pressure are in the loop 10-16, which contains the active site of BPTI, and residues 38-42, which are adjacent to buried water molecules. Hydrogen bonds ...[more]