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Late memory-related genes in the hippocampus revealed by RNA fingerprinting.

ABSTRACT: Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of the ryanodine receptor type-2 and glutamate dehydrogenase (EC 1.4.1.3), were further investigated by Northern blot analysis, reverse transcription-PCR, and in situ hybridization and confirmed as MRGs with distinct temporal and regional expression. Successive RNA screening as illustrated here may help to reveal a spectrum of MRGs as they appear in distinct domains of memory storage.

SUBMITTER: Cavallaro S

PROVIDER: S-EPMC23247 | biostudies-literature | 1997 Sep

REPOSITORIES: biostudies-literature

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Late memory-related genes in the hippocampus revealed by RNA fingerprinting.

Cavallaro S S Meiri N N Yi C L CL Musco S S Ma W W Goldberg J J Alkon D L DL

Proceedings of the National Academy of Sciences of the United States of America 19970901 18

Although long-term memory is thought to require a cellular program of gene expression and increased protein synthesis, the identity of proteins critical for associative memory is largely unknown. We used RNA fingerprinting to identify candidate memory-related genes (MRGs), which were up-regulated in the hippocampus of water maze-trained rats, a brain area that is critically involved in spatial learning. Two of the original 10 candidate genes implicated by RNA fingerprinting, the rat homolog of t ...[more]

PMID: 9275181

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