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A high-resolution structure of ligand-free human glutamate carboxypeptidase II.

ABSTRACT: Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an established marker for prostate-cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII-specific inhibitors and diagnostic probes. The currently available structure of ligand-free GCPII was refined to a resolution of 3.5 A. This work reports the structure of the protein refined to 1.65 A resolution, with crystallographic values of R = 0.207 and R(free) = 0.228. The new structure extends the resolution appreciably and the new model based on this data shows significant differences when compared with the previously published model.

SUBMITTER: Barinka C

PROVIDER: S-EPMC2330195 | biostudies-literature | 2007 Mar

REPOSITORIES: biostudies-literature

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A high-resolution structure of ligand-free human glutamate carboxypeptidase II.

Barinka Cyril C Starkova Jana J Konvalinka Jan J Lubkowski Jacek J

Acta crystallographica. Section F, Structural biology and crystallization communications 20070213 Pt 3

Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an established marker for prostate-cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII-specific inhibitors and diagnostic probes. The currently available structure of ligand-free GCPII was refined to a resolution of 3.5 A. This work reports the structure of the prot ...[more]

PMID: 17329803

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