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Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus.

ABSTRACT: Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 A. Amino-acid residues 1-371 of the 44 kDa protein were identified by Pfam as an HD domain and a member of the metal-dependent phosphohydrolase superfamily (accession No. PF01966). Although three families from this large and diverse group of enzymatic proteins are represented in the PDB, the structure of 067745_AQUAE reveals a unique fold that is unlike the others and that is likely to represent a new subfamily, further organizing the families and characterizing the proteins. Data are presented that provide the first insights into the structural organization of the proteins within this clan and a distal alternative GDP-binding domain outside the metal-binding active site is proposed.

SUBMITTER: Oganesyan V

PROVIDER: S-EPMC2335016 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature

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Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus.

Oganesyan Vaheh V Adams Paul D PD Jancarik Jarmila J Kim Rosalind R Kim Sung-Hou SH

Acta crystallographica. Section F, Structural biology and crystallization communications 20070428 Pt 5

Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 A. Amino-acid residues 1-371 of the 44 kDa protein were identified by Pfam as an HD domain and a member of the metal-dependent phosphohydrolase superfamily (accession No. PF01966). Although three families from this large and diverse group of enzymatic proteins a ...[more]

PMID: 17565173

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Crystal structure of conserved hypothetical protein Aq1575 from Aquifex aeolicus.

Project description:The crystal structure of a conserved hypothetical protein, Aq1575, from Aquifex aeolicus has been determined by using x-ray crystallography. The protein belongs to the domain of unknown function DUF28 in the Pfam and PALI databases for which there was no structural information available until now. A structural homology search with the DALI algorithm indicates that this protein has a new fold with no obvious similarity to those of other proteins of known three-dimensional structure. The protein reveals a monomer consisting of three domains arranged along a pseudo threefold symmetry axis. There is a large cleft with approximate dimensions of 10 A x 10 A x 20 A in the center of the three domains along the symmetry axis. Two possible active sites are suggested based on the structure and multiple sequence alignment. There are several highly conserved residues in these putative active sites. The structure based molecular properties and thermostability of the protein are discussed.

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Adenosine triphosphate stimulates Aquifex aeolicus MutL endonuclease activity.

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| S-EPMC2744016 | biostudies-literature

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