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Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase.

ABSTRACT: Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 A, alpha = beta = gamma = 90.0 degrees . Diffraction data were collected to 2.6 A resolution using synchrotron radiation at BL24XU of SPring-8.

SUBMITTER: Morita H

PROVIDER: S-EPMC2339743 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

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Crystallization and preliminary crystallographic analysis of an octaketide-producing plant type III polyketide synthase.

Morita Hiroyuki H Kondo Shin S Kato Ryohei R Wanibuchi Kiyofumi K Noguchi Hiroshi H Sugio Shigetoshi S Abe Ikuro I Kohno Toshiyuki T

Acta crystallographica. Section F, Structural biology and crystallization communications 20071024 Pt 11

Octaketide synthase (OKS) from Aloe arborescens is a plant-specific type III polyketide synthase that produces SEK4 and SEK4b from eight molecules of malonyl-CoA. Recombinant OKS expressed in Escherichia coli was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group I422, with unit-cell parameters a = b = 110.2, c = 281.4 A, alpha = beta = gamma = 90.0 degrees . Diffraction data were collected to 2.6 A resolution using synchrotron radiation at BL24XU of S ...[more]

PMID: 18007047

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