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Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.

ABSTRACT: HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 A resolution and the crystal belonged to the hexagonal space group P6(3). Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.

SUBMITTER: Nakamura T

PROVIDER: S-EPMC2344092 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.

Nakamura Toshio T Tonozuka Takashi T Kotani Mao M Obata Kanae K Oguma Keiji K Nishikawa Atsushi A

Acta crystallographica. Section F, Structural biology and crystallization communications 20071121 Pt 12

HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 A resolution and the crystal belonged to the hexagonal space group P6(3). Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric ...[more]

PMID: 18084087

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