Ontology highlight
ABSTRACT:
SUBMITTER: Koc EC
PROVIDER: S-EPMC2374141 | biostudies-literature | 2001 Mar
REPOSITORIES: biostudies-literature
Koc E C EC Burkhart W W Blackburn K K Koc H H Moseley A A Spremulli L L LL
Protein science : a publication of the Protein Society 20010301 3
Proteins in the small subunit of the mammalian mitochondrial ribosome were separated by two-dimensional polyacrylamide gel electrophoresis. Four individual proteins were subjected to in-gel Endoprotease Lys-C digestion. The sequences of selected proteolytic peptides were obtained by electrospray tandem mass spectrometry. Peptide sequences obtained from in-gel digestion of individual spots were used to screen human, mouse, and rat expressed sequence tag databases, and complete consensus cDNAs for ...[more]