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ABSTRACT:
SUBMITTER: Zhang Y
PROVIDER: S-EPMC2374155 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Acta crystallographica. Section F, Structural biology and crystallization communications 20080229 Pt 3
AaManA, a beta-1,4-mannanase from the thermoacidophile Alicyclobacillus acidocaldarius Tc-12-31, was expressed in Escherichia coli and purified in a form suitable for X-ray crystallographic analysis. Crystals were obtained using the hanging-drop vapour-diffusion method at 291 K using ammonium dihydrogen phosphate as a precipitant. Data were collected from native mannanase and from a selenomethionyl derivative to 1.90 and 1.99 A, respectively, at 100 K. The native crystal belongs to the orthorhom ...[more]