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ABSTRACT:
SUBMITTER: Igura M
PROVIDER: S-EPMC2376324 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Igura Mayumi M Maita Nobuo N Obita Takayuki T Kamishikiryo Jun J Maenaka Katsumi K Kohda Daisuke D
Acta crystallographica. Section F, Structural biology and crystallization communications 20070831 Pt 9
Oligosaccharyltransferase catalyzes the transfer of preassembled oligosaccharides onto asparagine residues in nascent polypeptide chains. The STT3 subunit is thought to bear the catalytic site. The C-terminal domain of the STT3 protein of Pyrococcus furiosus was expressed in Escherichia coli cells. STT3 protein prepared from two different sources, the soluble fraction and the inclusion bodies, produced crystals that diffracted to 2.7 A. During crystallization screening, cocrystals of P. furiosus ...[more]