Tractable Cre-lox system for stochastic alteration of genes in mice.
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ABSTRACT:
SUBMITTER: Miller AJ
PROVIDER: S-EPMC2394730 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Publications
Tractable Cre-lox system for stochastic alteration of genes in mice.
Miller Ashleigh J AJ Dudley Sandra D SD Tsao Jen-Lan JL Shibata Darryl D Liskay R Michael RM
Nature methods 20080210 3
We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations. ...[more]
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