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Rapid species identification of cooked poisonous mushrooms by using real-time PCR.


ABSTRACT: Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h.

SUBMITTER: Maeta K 

PROVIDER: S-EPMC2394946 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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Rapid species identification of cooked poisonous mushrooms by using real-time PCR.

Maeta Kazuhiko K   Ochi Tomoya T   Tokimoto Keisuke K   Shimomura Norihiro N   Maekawa Nitaro N   Kawaguchi Nobuhisa N   Nakaya Makoto M   Kitamoto Yutaka Y   Aimi Tadanori T  

Applied and environmental microbiology 20080331 10


Species-specific identification of the major cooked and fresh poisonous mushrooms in Japan was performed using a real-time PCR system. Specific fluorescence signals were detected, and no nonspecific signals were detected. Therefore, we succeeded in developing a species-specific test for the identification of poisonous mushrooms within 1.5 h. ...[more]

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