Transcription factors Pcr1 and Atf1 have distinct roles in stress- and Sty1-dependent gene regulation.

ABSTRACT: The mitogen-activated protein kinase Sty1 is essential for the regulation of transcriptional responses that promote cell survival in response to different types of environmental stimuli in Schizosaccharomyces pombe. Upon stress activation, Sty1 reversibly accumulates in the nucleus, where it stimulates gene expression via the Atf1 transcription factor. The Atf1 protein forms a heterodimer with Pcr1, but the specific role of this association is controversial. We have carried out a comparative analysis of strains lacking these proteins individually. We demonstrate that Atf1 and Pcr1 have similar but not identical roles in S. pombe, since cells lacking Pcr1 do not share all the phenotypes reported for Deltaatf1 cells. Northern blot and microarray analyses demonstrate that the responses to spe

SUBMITTER: Sanso M 

PROVIDER: S-EPMC2394965 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature