HIV-1 Vif promotes the formation of high molecular mass APOBEC3G complexes.
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ABSTRACT:
SUBMITTER: Goila-Gaur R
PROVIDER: S-EPMC2396953 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Publications
HIV-1 Vif promotes the formation of high molecular mass APOBEC3G complexes.
Goila-Gaur Ritu R Khan Mohammad A MA Miyagi Eri E Kao Sandra S Opi Sandrine S Takeuchi Hiroaki H Strebel Klaus K
Virology 20071119 1
HIV-1 Vif inhibits the antiviral activity of APOBEC3G (APO3G) by inducing proteasomal degradation. Here, we studied the effects of Vif on APO3G in vitro. In this system, Vif did not cause APO3G degradation. Instead, Vif induced changes in APO3G that affected immunoprecipitation of the native protein. This effect required wt Vif and was reversed by heat denaturation of APO3G. Sucrose gradient analysis demonstrated that wt Vif induced the gradual transition of APO3G translated in vitro or expresse ...[more]
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