Ontology highlight
ABSTRACT:
SUBMITTER: Camilloni C
PROVIDER: S-EPMC2397339 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Camilloni C C Rocco A Guerini AG Eberini I I Gianazza E E Broglia R A RA Tiana G G
Biophysical journal 20080313 12
In performing protein-denaturation experiments, it is common to employ different kinds of denaturants interchangeably. We make use of molecular dynamics simulations of Protein L in water, in urea, and in guanidinium chloride (GdmCl) to ascertain if there are any structural differences in the associated unfolding processes. The simulation of proteins in solutions of GdmCl is complicated by the large number of charges involved, making it difficult to set up a realistic force field. Furthermore, at ...[more]