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Gene expression-based screening for inhibitors of PDGFR signaling.

ABSTRACT: Here we describe a proof-of-concept experiment designed to explore the possibility of using gene expression-based high-throughput screening (GE-HTS) to find inhibitors of a signaling cascade, using platelet derived growth factor receptor (PDGFR) signaling as the example. The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small molecule modulators of any signaling pathway of interest.

SUBMITTER: Antipova AA

PROVIDER: S-EPMC2397499 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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Gene expression-based screening for inhibitors of PDGFR signaling.

Antipova Alena A AA Stockwell Brent R BR Golub Todd R TR

Genome biology 20080301 3

Here we describe a proof-of-concept experiment designed to explore the possibility of using gene expression-based high-throughput screening (GE-HTS) to find inhibitors of a signaling cascade, using platelet derived growth factor receptor (PDGFR) signaling as the example. The previously unrecognized ability of aurintricarboxylic acid to inhibit PDGFR signaling, discovered through a screen of 1,739 compounds, demonstrates the feasibility and generalizability of GE-HTS for the discovery of small mo ...[more]

PMID: 18312689

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