DNA signatures for detecting genetic engineering in bacteria.
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ABSTRACT:
SUBMITTER: Allen JE
PROVIDER: S-EPMC2397508 | biostudies-literature | 2008
REPOSITORIES: biostudies-literature
Publications
DNA signatures for detecting genetic engineering in bacteria.
Allen Jonathan E JE Gardner Shea N SN Slezak Tom R TR
Genome biology 20080318 3
Using newly designed computational tools we show that, despite substantial shared sequences between natural plasmids and artificial vector sequences, a robust set of DNA oligomers can be identified that can differentiate artificial vector sequences from all available background viral and bacterial genomes and natural plasmids. We predict that these tools can achieve very high sensitivity and specificity rates for detecting new unsequenced vectors in microarray-based bioassays. Such DNA signature ...[more]
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