Ontology highlight
ABSTRACT:
SUBMITTER: Batra VK
PROVIDER: S-EPMC2399898 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Batra Vinod K VK Beard William A WA Shock David D DD Pedersen Lars C LC Wilson Samuel H SH
Molecular cell 20080501 3
We report the crystallographic structures of DNA polymerase beta with dG-dAMPCPP and dC-dAMPCPP mismatches in the active site. These premutagenic structures were obtained with a nonhydrolyzable incoming nucleotide analog, dAMPCPP, and Mn(2+). Substituting Mn(2+) for Mg(2+) significantly decreases the fidelity of DNA synthesis. The structures reveal that the enzyme is in a closed conformation like that observed with a matched Watson-Crick base pair. The incorrect dAMPCPP binds in a conformation i ...[more]