Ontology highlight
ABSTRACT:
SUBMITTER: Vazquez-Ibar JL
PROVIDER: S-EPMC240682 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Vázquez-Ibar José Luis JL Guan Lan L Svrakic Maja M Kaback H Ronald HR
Proceedings of the National Academy of Sciences of the United States of America 20031017 22
The crystal structure of the Escherichia coli lactose permease at 3.5 A with a bound substrate has been reported recently. The structure reveals the sugar-protein contacts, which include hydrophobic stacking between the galactopyranosyl ring of substrate and the indole side chain of Trp-151, as proposed previously. The nature of this interaction is studied here by exploiting the luminescence properties of Trp-151 in a mutant devoid of other tryptophan residues. The following phenomena are observ ...[more]