Ontology highlight
ABSTRACT:
SUBMITTER: Hitchcock AL
PROVIDER: S-EPMC240687 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Hitchcock Amy L AL Auld Kathryn K Gygi Steven P SP Silver Pamela A PA
Proceedings of the National Academy of Sciences of the United States of America 20031013 22
Ubiquitination of membrane-associated proteins can direct their proteasome-mediated degradation or activation at the endoplasmic reticulum (ER), as well as their endocytosis and intracellular sorting. However, the full spectrum of ubiquitinated membrane proteins has not been determined. Here we combined proteomic analysis with yeast genetics to identify 211 ubiquitinated membrane-associated proteins in Saccharomyces cerevisiae and map >30 precise sites of ubiquitination. Major classes of identif ...[more]