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The structural basis for the oriented assembly of a TBP/TFB/promoter complex.

ABSTRACT: Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription.

SUBMITTER: Littlefield O

PROVIDER: S-EPMC24122 | biostudies-literature | 1999 Nov

REPOSITORIES: biostudies-literature

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The structural basis for the oriented assembly of a TBP/TFB/promoter complex.

Littlefield O O Korkhin Y Y Sigler P B PB

Proceedings of the National Academy of Sciences of the United States of America 19991101 24

Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promote ...[more]

PMID: 10570130

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