Ontology highlight
ABSTRACT:
SUBMITTER: Turunen JJ
PROVIDER: S-EPMC2423181 | biostudies-literature | 2008 May
REPOSITORIES: biostudies-literature
Turunen Janne J JJ Will Cindy L CL Grote Michael M Lührmann Reinhard R Frilander Mikko J MJ
Molecular and cellular biology 20080317 10
Little is currently known about proteins that make contact with the pre-mRNA in the U12-dependent spliceosome and thereby contribute to intron recognition. Using site-specific cross-linking, we detected an interaction between the U11-48K protein and U12-type 5' splice sites (5'ss). This interaction did not require branch point recognition and was sensitive to 5'ss mutations, suggesting that 48K interacts with the 5'ss during the first steps of prespliceosome assembly in a sequence-dependent mann ...[more]