Ontology highlight
ABSTRACT:
SUBMITTER: Buchser WJ
PROVIDER: S-EPMC2424126 | biostudies-literature | 2006 Nov
REPOSITORIES: biostudies-literature
Buchser William J WJ Pardinas Jose R JR Shi Yan Y Bixby John L JL Lemmon Vance P VP
BioTechniques 20061101 5
Manipulating gene expression in primary neurons has been a goal for many scientists for over 20 years. Vertebrate central nervous system neurons are classically difficult to transfect. Most lipid reagents are inefficient and toxic to the cells, and time-consuming methods such as viral infections are often required to obtain better efficiencies. We have developed an efficient method for the transfection of cerebellar granule neurons and hippocampal neurons with standard plasmid vectors. Using 96- ...[more]