Developmentally programmed DNA splicing in Paramecium reveals short-distance crosstalk between DNA cleavage sites.

ABSTRACT: Somatic genome assembly in the ciliate Paramecium involves the precise excision of thousands of short internal eliminated sequences (IESs) that are scattered throughout the germline genome and often interrupt open reading frames. Excision is initiated by double-strand breaks centered on the TA dinucleotides that are conserved at each IES boundary, but the factors that drive cleavage site recognition remain unknown. A degenerate consensus was identified previously at IES ends and genetic analyses confirmed the participation of their nucleotide sequence in efficient excision. Even for wild-type IESs, however, variant excision patterns (excised or nonexcised) may be inherited maternally through sexual events, in a homology-dependent manner. We show here that this maternal epigenetic control i

SUBMITTER: Gratias A 

PROVIDER: S-EPMC2425466 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature