Ontology highlight
ABSTRACT:
SUBMITTER: Rust MJ
PROVIDER: S-EPMC2427396 | biostudies-literature | 2007 Nov
REPOSITORIES: biostudies-literature
Rust Michael J MJ Markson Joseph S JS Lane William S WS Fisher Daniel S DS O'Shea Erin K EK
Science (New York, N.Y.) 20071004 5851
The simple circadian oscillator found in cyanobacteria can be reconstituted in vitro using three proteins-KaiA, KaiB, and KaiC. The total phosphorylation level of KaiC oscillates with a circadian period, but the mechanism underlying its sustained oscillation remains unclear. We have shown that four forms of KaiC differing in their phosphorylation state appear in an ordered pattern arising from the intrinsic autokinase and autophosphatase rates of KaiC and their modulation by KaiA. Kinetic and bi ...[more]