Ontology highlight
ABSTRACT:
SUBMITTER: Dupasquier M
PROVIDER: S-EPMC2430420 | biostudies-literature | 2008 Jun
REPOSITORIES: biostudies-literature
Dupasquier Marcel M Kim Sangbumn S Halkidis Konstantine K Gamper Howard H Hou Ya-Ming YM
Journal of molecular biology 20080408 3
CCA addition to the 3' end is an essential step in tRNA maturation. High-resolution crystal structures of the CCA enzymes reveal primary enzyme contact with the tRNA minihelix domain, consisting of the acceptor stem and T stem-loop. RNA and DNA minihelices are efficient substrates for CCA addition in steady-state kinetics. However, in contrast to structural models and steady-state experiments, we show here by single-turnover kinetics that minihelices are insufficient substrates for the Escherich ...[more]