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Extra centrosomes and/or chromosomes prolong mitosis in human cells.

ABSTRACT: Using laser microsurgery and cell fusion we have explored how additional centrosomes and/or chromosomes influence the duration of mitosis in human cells. We found that doubling the chromosome number added approximately 10 min to a 20 min division, whereas doubling the number of centrosomes added approximately 30 min more. Extra centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the spindle assembly checkpoint. Thus mitosis can be prolonged by non-genetic means and extra chromosomes and centrosomes probably contribute to the elevated mitotic index seen in many tumours.

SUBMITTER: Yang Z

PROVIDER: S-EPMC2430725 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

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Extra centrosomes and/or chromosomes prolong mitosis in human cells.

Yang Zhenye Z Loncarek Jadranka J Khodjakov Alexey A Rieder Conly L CL

Nature cell biology 20080511 6

Using laser microsurgery and cell fusion we have explored how additional centrosomes and/or chromosomes influence the duration of mitosis in human cells. We found that doubling the chromosome number added approximately 10 min to a 20 min division, whereas doubling the number of centrosomes added approximately 30 min more. Extra centrosomes and/or chromosomes prolong mitosis by delaying satisfaction of the spindle assembly checkpoint. Thus mitosis can be prolonged by non-genetic means and extra c ...[more]

PMID: 18469805

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Extra centrosomes induce PIDD1-mediated inflammation and immunosurveillance

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Nucleoporin Nup58 localizes to centrosomes and mid-bodies during mitosis.

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| S-EPMC6679547 | biostudies-literature

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