Unknown

Dataset Information

0

Btn1 affects endocytosis, polarization of sterol-rich membrane domains and polarized growth in Schizosaccharomyces pombe.


ABSTRACT: btn1, the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3, exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37 degrees C that culminate in total depolarized growth and cell lysis. These defects are preceded by a progressive failure to correctly polarize sterol-rich domains after cytokinesis and are accompanied by loss of Myo1p localization. Furthermore, we found that in Sz. pombe, sterol spreading is linked to defective formation/polarization of F-actin patches and disruption of endocytosis and that these processes are aberrant in btn1Delta cells. Consistent with a role for Btn1p in polarized growth, Btn1p has an altered location at 37 degrees C and is retained in actin-dependent endomembrane structures near the cell poles or septum.

SUBMITTER: Codlin S 

PROVIDER: S-EPMC2440566 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2900468 | biostudies-literature
| S-EPMC2946365 | biostudies-literature
| S-EPMC25432 | biostudies-literature
| S-EPMC4132169 | biostudies-literature
| S-EPMC3487762 | biostudies-literature
| S-EPMC6078436 | biostudies-literature
| S-EPMC3873978 | biostudies-literature
| S-EPMC11196315 | biostudies-literature
| S-EPMC2643608 | biostudies-literature
| S-EPMC6893373 | biostudies-literature