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The genome sequence of the model ascomycete fungus Podospora anserina.

ABSTRACT: BACKGROUND: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development. RESULTS: We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina genome and orthologous gene set with the one of its close relatives, Neurospora crassa, shows that synteny is poorly conserved, the main result of evolution being gene shuffling in the same chromosome. The P. anserina genome contains fewer repeated sequences and has evolved new genes by duplication since its separation from N. crassa, despite the presence of the repeat induced point mutation mechanism that mutates duplicated sequences. We also provide evidence that frequent gene loss took place in the lineages leading to P. anserina and N. crassa. P. anserina contains a large and highly specialized set of genes involved in utilization of natural carbon sources commonly found in its natural biotope. It includes genes potentially involved in lignin degradation and efficient cellulose breakdown. CONCLUSION: The features of the P. anserina genome indicate a highly dynamic evolution since the divergence of P. anserina and N. crassa, leading to the ability of the former to use specific complex carbon sources that match its needs in its natural biotope.

SUBMITTER: Espagne E

PROVIDER: S-EPMC2441463 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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The genome sequence of the model ascomycete fungus Podospora anserina.

Espagne Eric E Lespinet Olivier O Malagnac Fabienne F Da Silva Corinne C Jaillon Olivier O Porcel Betina M BM Couloux Arnaud A Aury Jean-Marc JM Ségurens Béatrice B Poulain Julie J Anthouard Véronique V Grossetete Sandrine S Khalili Hamid H Coppin Evelyne E Déquard-Chablat Michelle M Picard Marguerite M Contamine Véronique V Arnaise Sylvie S Bourdais Anne A Berteaux-Lecellier Véronique V Gautheret Daniel D de Vries Ronald P RP Battaglia Evy E Coutinho Pedro M PM Danchin Etienne Gj EG Henrissat Bernard B Khoury Riyad El RE Sainsard-Chanet Annie A Boivin Antoine A Pinan-Lucarré Bérangère B Sellem Carole H CH Debuchy Robert R Wincker Patrick P Weissenbach Jean J Silar Philippe P

Genome biology 20080506 5

<h4>Background</h4>The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.<h4>Results</h4>We present a 10X draft sequence of P. anserina genome, linked to the sequences of a large expressed sequence tag collection. Similar to higher eukaryotes, the P. anserina transcription/splicing machinery generates numerous non-conventional transcripts. Comparison of the P. anserina geno ...[more]

PMID: 18460219

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Project description:BackgroundThe ascomycete fungus Podospora anserina has been appreciated for its targeted carbohydrate-active enzymatic arsenal. As a late colonizer of herbivorous dung, the fungus acts specifically on the more recalcitrant fraction of lignocellulose and this lignin-rich biotope might have resulted in the evolution of ligninolytic activities. However, the lignin-degrading abilities of the fungus have not been demonstrated by chemical analyses at the molecular level and are, thus far, solely based on genome and secretome predictions. To evaluate whether P. anserina might provide a novel source of lignin-active enzymes to tap into for potential biotechnological applications, we comprehensively mapped wheat straw lignin during fungal growth and characterized the fungal secretome.ResultsQuantitative 13C lignin internal standard py-GC-MS analysis showed substantial lignin removal during the 7 days of fungal growth (24% w/w), though carbohydrates were preferably targeted (58% w/w removal). Structural characterization of residual lignin by using py-GC-MS and HSQC NMR analyses demonstrated that C?-oxidized substructures significantly increased through fungal action, while intact ?-O-4' aryl ether linkages, p-coumarate and ferulate moieties decreased, albeit to lesser extents than observed for the action of basidiomycetes. Proteomic analysis indicated that the presence of lignin induced considerable changes in the secretome of P. anserina. This was particularly reflected in a strong reduction of cellulases and galactomannanases, while H2O2-producing enzymes clearly increased. The latter enzymes, together with laccases, were likely involved in the observed ligninolysis.ConclusionsFor the first time, we provide unambiguous evidence for the ligninolytic activity of the ascomycete fungus P. anserina and expand the view on its enzymatic repertoire beyond carbohydrate degradation. Our results can be of significance for the development of biological lignin conversion technologies by contributing to the quest for novel lignin-active enzymes and organisms.

Dataset Information

The genome sequence of the model ascomycete fungus Podospora anserina.

Publications

The genome sequence of the model ascomycete fungus Podospora anserina.

Similar Datasets

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