High throughput automated allele frequency estimation by pyrosequencing.
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ABSTRACT:
SUBMITTER: Doostzadeh J
PROVIDER: S-EPMC2442187 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Publications
High throughput automated allele frequency estimation by pyrosequencing.
Doostzadeh Julie J Shokralla Shadi S Absalan Farnaz F Jalili Roxana R Mohandessi Sharareh S Langston James W JW Davis Ronald W RW Ronaghi Mostafa M Gharizadeh Baback B
PloS one 20080716 7
Pyrosequencing is a DNA sequencing method based on the principle of sequencing-by-synthesis and pyrophosphate detection through a series of enzymatic reactions. This bioluminometric, real-time DNA sequencing technique offers unique applications that are cost-effective and user-friendly. In this study, we have combined a number of methods to develop an accurate, robust and cost efficient method to determine allele frequencies in large populations for association studies. The assay offers the adva ...[more]
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