Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis.

ABSTRACT: Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein-yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microsc

SUBMITTER: Goss JW 

PROVIDER: S-EPMC2442215 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature