Practical method for detection and identification of Candida, Aspergillus, and Scedosporium spp. by use of rolling-circle amplification.
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ABSTRACT:
SUBMITTER: Zhou X
PROVIDER: S-EPMC2446935 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Publications
Practical method for detection and identification of Candida, Aspergillus, and Scedosporium spp. by use of rolling-circle amplification.
Zhou Xiaoyong X Kong Fanrong F Sorrell Tania C TC Wang Hui H Duan Yiqun Y Chen Sharon C A SC
Journal of clinical microbiology 20080521 7
A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and six of Scedosporium spp.), all were all identified correctly. ...[more]
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