Ontology highlight
ABSTRACT:
SUBMITTER: Roca I
PROVIDER: S-EPMC2447006 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Roca Ignasi I Torrents Eduard E Sahlin Margareta M Gibert Isidre I Sjöberg Britt-Marie BM
Journal of bacteriology 20080523 14
The Streptococcus pyogenes genome harbors two clusters of class Ib ribonucleotide reductase genes, nrdHEF and nrdF*I*E*, and a second stand-alone nrdI gene, designated nrdI2. We show that both clusters are expressed simultaneously as two independent operons. The NrdEF enzyme is functionally active in vitro, while the NrdE*F* enzyme is not. The NrdF* protein lacks three of the six highly conserved iron-liganding side chains and cannot form a dinuclear iron site or a tyrosyl radical. In vivo, on t ...[more]