Ontology highlight
ABSTRACT:
SUBMITTER: Lee SJ
PROVIDER: S-EPMC2449338 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Lee Seung-Joo SJ Qimron Udi U Richardson Charles C CC
Proceedings of the National Academy of Sciences of the United States of America 20080623 26
The DNA helicase encoded by bacteriophage T7 consists of six identical subunits that form a ring through which the DNA passes. Binding of ssDNA is a prior step to translocation and unwinding of DNA by the helicase. Arg-493 is located at a conserved structural motif within the interior cavity of the helicase and plays an important role in DNA binding. Replacement of Arg-493 with lysine or histidine reduces the ability of the helicase to bind DNA, hydrolyze dTTP, and unwind dsDNA. In contrast, rep ...[more]