Unknown

Dataset Information

0

Alterations in mucosal immunity identified in the colon of patients with irritable bowel syndrome.


ABSTRACT:

Background & aims

Irritable bowel syndrome (IBS) has been associated with mucosal dysfunction, mild inflammation, and altered colonic bacteria. We used microarray expression profiling of sigmoid colon mucosa to assess whether there are stably expressed sets of genes that suggest there are objective molecular biomarkers associated with IBS.

Methods

Gene expression profiling was performed using Human Genome U133 Plus 2.0 (Affymetrix) GeneChips with RNA from sigmoid colon mucosal biopsy specimens from 36 IBS patients and 25 healthy control subjects. Real-time quantitative polymerase chain reaction was used to confirm the data in 12 genes of interest. Statistical methods for microarray data were applied to search for differentially expressed genes, and to assess the stability of molecular signatures in IBS patients.

Results

Mucosal gene expression profiles were consistent across different sites within the sigmoid colon and were stable on repeat biopsy over approximately 3 months. Differentially expressed genes suggest functional alterations of several components of the host mucosal immune response to microbial pathogens. The most strikingly increased expression involved a yet uncharacterized gene, DKFZP564O0823. Identified specific genes suggest the hypothesis that molecular signatures may enable distinction of a subset of IBS patients from healthy controls. By using 75% of the biopsy specimens as a validation set to develop a gene profile, the test set (25%) was predicted correctly with approximately 70% accuracy.

Conclusions

Mucosal gene expression analysis shows there are relatively stable alterations in colonic mucosal immunity in IBS. These molecular alterations provide the basis to test the hypothesis that objective biomarkers may be identified in IBS and enhance understanding of the disease.

SUBMITTER: Aerssens J 

PROVIDER: S-EPMC2453689 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Alterations in mucosal immunity identified in the colon of patients with irritable bowel syndrome.

Aerssens Jeroen J   Camilleri Michael M   Talloen Willem W   Thielemans Leen L   Göhlmann Hinrich W H HW   Van Den Wyngaert Ilse I   Thielemans Theo T   De Hoogt Ronald R   Andrews Christopher N CN   Bharucha Adil E AE   Carlson Paula J PJ   Busciglio Irene I   Burton Duane D DD   Smyrk Thomas T   Urrutia Raul R   Coulie Bernard B  

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association 20080201 2


<h4>Background & aims</h4>Irritable bowel syndrome (IBS) has been associated with mucosal dysfunction, mild inflammation, and altered colonic bacteria. We used microarray expression profiling of sigmoid colon mucosa to assess whether there are stably expressed sets of genes that suggest there are objective molecular biomarkers associated with IBS.<h4>Methods</h4>Gene expression profiling was performed using Human Genome U133 Plus 2.0 (Affymetrix) GeneChips with RNA from sigmoid colon mucosal bio  ...[more]

Similar Datasets

| S-EPMC6109711 | biostudies-literature
2007-11-24 | E-TABM-176 | biostudies-arrayexpress
| S-EPMC5076008 | biostudies-literature
| S-EPMC1774694 | biostudies-literature
| S-EPMC7098960 | biostudies-literature
| S-EPMC6650632 | biostudies-literature
| S-EPMC8590216 | biostudies-literature
| S-EPMC6068233 | biostudies-literature
| S-EPMC1891510 | biostudies-other
| S-EPMC8603515 | biostudies-literature