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A genome-wide view of the spectrum of spontaneous mutations in yeast.

ABSTRACT: The mutation process ultimately defines the genetic features of all populations and, hence, has a bearing on a wide range of issues involving evolutionary genetics, inheritance, and genetic disorders, including the predisposition to cancer. Nevertheless, formidable technical barriers have constrained our understanding of the rate at which mutations arise and the molecular spectrum of their effects. Here, we report on the use of complete-genome sequencing in the characterization of spontaneously arising mutations in the yeast Saccharomyces cerevisiae. Our results confirm some findings previously obtained by indirect methods but also yield numerous unexpected findings, in particular a very high rate of point mutation and skewed distribution of base-substitution types in the mitochondrion, a very high rate of segmental duplication and deletion in the nuclear genome, and substantial deviations in the mutational profile among various model organisms.

SUBMITTER: Lynch M

PROVIDER: S-EPMC2453693 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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PROTEIN HAVING FUNCTION TO CONTROL LOCALIZATION OF LIPOPROTEIN IN CELL OF MICROORGANISM BELONGING TO GRAM- NEGATIVE BACTERIUM AND DNA ENCODING THE SAME

TOKUDA HAJIME

20000224

PROBLEM TO BE SOLVED: To identify and provide proteins each having such a general function as to isolate from cell membrane and deliver to localization mechanism (LolA- LolB mechanism) to outer membrane a matured lipoprotein after processing in microorganisms belonging to Gram-negative bacteria. SOLUTION: The protein, proteins (LolC, LolD and LolE) each having the function to control the localization of a lipoprotein in Escherichia coli, are identified, and their amino acid sequences are identif ...[more]

PMID: JP2001231570

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