Multiple cellular mechanisms related to cyclin A1 in prostate cancer invasion and metastasis.

ABSTRACT:

Background

Cyclin A1 is a cell cycle regulator that has been implicated in the progression of prostate cancer. Its role in invasion and metastasis of this disease has not been characterized.

Methods

Immunohistochemistry and cDNA microarray analyses were used to assess protein and mRNA expression of cyclin A1 and proteins with roles in metastasis, including vascular endothelial growth factor (VEGF), metalloproteinase 2 (MMP2), and MMP9, in human prostate cancer. Transient transfection and infection with viral vectors expressing cyclin A1 and short hairpin RNA (shRNA) targeting cyclin A1 were used to study the effects of altered cyclin A1 expression in PC3 prostate cancer cells. The BrdU assay, annexin V staining, and invasion chambers were used to examine cyclin A1 effects on

SUBMITTER: Wegiel B 

PROVIDER: S-EPMC2467435 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature